Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide :: GCSE Chemistry Coursework Investigation

Investigate the Effect of pH on Immobilised barm Cells on the Breakdown of heat content peroxideSafety=======* Extreme basic and acids are utilize so moldiness wear gloves, goggles and apron.* Be actually careful not to make contact with any of the chemicals, as they leave behind be irritable and some corrosive to the skin and eyes.* at that place must be no running and any other conventionalism research laboratory rules should be followed as usual.* Hydrogen peroxide is corrosive and so should not be touched.* A lab technician should immediately wash up spillages.* ever much be careful of the glass instruments as these could break.Background association In my experiment I pull up stakes be measuring the metreof type O given of in a gas syringe everyplace a certain time period fromdifferent pH levels.Hydrogen Peroxide is a chemical compound, H2O2, a colourless, syrupyliquid that is a laborious oxidizing agent and an in water resolution a gutless acid. It is comp atible with cold water and is soluble in alcoholand ether. Although comminuted hydrogen peroxide is fairly stable, itdecomposes into water and oxygen when heated supra active 80C it in any casedecomposes in the presence of numerous throttle valves e.g. more or less metals,acids.The properties and factors that effect breakdown of Hydrogen Peroxideare definitive in this investigation. The reaction of Hydrogen Peroxideforms water and oxygen, which is shown in the comparison below.2H2O2 2H2O + O2On its own this reaction is very slow and unremarkably a catalyst isneeded to despatch up the reaction. A catalyst is used to speed upreactions without it getting used up. So a biological catalyst such asan enzyme is used to speed up the crop in the equation above. In myexperiment I am personnel casualty to do I am using dried yeast, which contains a satisfactory enzyme catalase. So therefore I am using the catalase inthe yeast to speed up the reaction, the products being produced an dthe overall results.To explain why enzymes speed up reactions it is based on the collisiontheory. The enzymes, which are immobilised in the yeast-based bead, depart give many active sites for the substrate (Hydrogen Peroxide) tobind ( be quiet) with. This will increase the collisions and thereforethe rate of reaction.Immobilised enzymes can be water-washed and re-used. The beads are allrelatively the same size so that there is less chance of error on theinvestigation. Immobilised enzymes are also more stable in extremes oftemperature and pH, which it will need for my experiment.A buffer zone solution is one, which resists changes in pH when smallInvestigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide GCSE interpersonal chemistry Coursework InvestigationInvestigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen PeroxideSafety=======* Extreme alkaline and acids are used so must wear gloves, goggles and apron.* Be v ery careful not to make contact with any of the chemicals, as they will be irritable and some corrosive to the skin and eyes.* There must be no running and any other normal laboratory rules should be followed as usual.* Hydrogen peroxide is corrosive and so should not be touched.* A lab technician should immediately wash up spillages.* Always be careful of the glass instruments as these could break.Background knowledge In my experiment I will be measuring the amountof oxygen given of in a gas syringe over a certain time period fromdifferent pH levels.Hydrogen Peroxide is a chemical compound, H2O2, a colourless, syrupyliquid that is a strong oxidizing agent and an in water solution aweak acid. It is miscible with cold water and is soluble in alcoholand ether. Although pure hydrogen peroxide is fairly stable, itdecomposes into water and oxygen when heated above about 80C it alsodecomposes in the presence of numerous catalysts e.g. most metals,acids.The properties and factors that ef fect breakdown of Hydrogen Peroxideare important in this investigation. The reaction of Hydrogen Peroxideforms water and oxygen, which is shown in the equation below.2H2O2 2H2O + O2On its own this reaction is very slow and normally a catalyst isneeded to speed up the reaction. A catalyst is used to speed upreactions without it getting used up. So a biological catalyst such asan enzyme is used to speed up the process in the equation above. In myexperiment I am going to do I am using dried yeast, which contains asuitable enzyme catalase. So therefore I am using the catalase inthe yeast to speed up the reaction, the products being produced andthe overall results.To explain why enzymes speed up reactions it is based on the collisiontheory. The enzymes, which are immobilised in the yeast-based beads,will give many active sites for the substrate (Hydrogen Peroxide) tobind (lock) with. This will increase the collisions and thereforethe rate of reaction.Immobilised enzymes can be washed and re-used. The beads are allrelatively the same size so that there is less chance of error on theinvestigation. Immobilised enzymes are also more stable in extremes oftemperature and pH, which it will need for my experiment.A buffer solution is one, which resists changes in pH when small